The leaders of the Democratic Unionist Party have demanded that Rishi Sunak stop building work on the new customs checkpoint in Northern Ireland.
A group of ex-ministers, including Jacob Rees-Mogg, have called on the Government to stop work at the ports.
The collapse of the powersharing institutions at Stormont was caused by the Democratic Unionist Party withdrawing co-operation.
The responsibility for constructing new facilities at Northern Ireland ports to check goods entering Northern Ireland from the rest of the UK falls on the Department for Environment, Food and Rural Affairs.
Legislation was published last month to make sure the facilities are completed.
In an early day motion tabled by the leader of the Democratic Unionist Party, Sir Jeffrey Donaldson, 14 MPs said that a customs border that divides the United Kingdom would be treated like a foreign country.
No10 played down hopes that a deal was close, as Rishi Sunak tried to soothe the nerves of the MPs.
The UK and EU are said to be close to a deal that would end years of wrangling.
The package includes scrapping routine checks on products destined for the province, according to reports.
If the domestic courts refer issues, the European Court of Justice can only intervene.
It is not certain that the terms will be acceptable to unionists in Northern Ireland.
The government is facing a legal challenge over legislation.
The statutory instrument that allows the completion of infrastructure for agri-food checks is against the law because it treats Northern Ireland as an entry point into the European Union.
The Northern Ireland Protocol claims that Northern Ireland is part of the customs territory of the United Kingdom, according to his claim.
The letter was sent to the Department for Environment, Food and Rural Affairs.
There are new customs and regulatory barriers between Northern Ireland and the rest of the UK as a result of the protocol.
Issues with the operation of the protocol are still being worked on.
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